Fig. 7

hes2 is required for proper hair cell polarity through regulation of Emx2.Phalloidin staining of 5 dpf neuromasts from hes2+/+ (A) and hes2−/− (B) Scale bar = 10 µm. C Quantification of posterior facing hair cells per neuromast. Data are presented as mean values  + /− 95% confidence intervals. ****p < 0.0001, two-tailed t-test. n = 37 for hes2+/+ and n = 47 for hes2−/−. D Quantification of hair cell number in 5 dpf neuromasts from hes2+/+ and hes2−/−. Data are presented as mean values  + /− 95% confidence intervals. ns = not significant p = 0.12, two-tailed t-test. n = 19 for hes2+/+ and n = 37 for hes2−/−. Anti-Emx2 immunostaining (yellow) in myo6b:H2B-mScarlet-I (magenta) hes2+/+ (E) and hes2-/− (F) 5dpf neuromasts. Scale bar = 10 µm. G Quantification of the percentage of Emx2+ hair cells per neuromast at 5 dpf. Data are presented as mean values  + /− 95% confidence intervals. ***p = 0.0009, two-tailed t-test. n = 14 for hes2+/+ and n = 20 for hes2−/−. H–K Phalloidin staining of 5 dpf neuromasts in control or ccndx:hes2.2 expressing, ccndx+/+ or ccndx−/−. Scale bar = 10 µm. L Quantification of posterior facing hair cells per neuromast. Data are presented as mean values  + /− 95% confidence intervals. ****p < 0.0001, Two-way Anova (genotype, condition) with Šidák multiple comparisons test. n = 20 for ccndx+/+/hes2-, n = 16 for ccndx+/+/hes2+, n = 24 for ccndx−/−/hes2- and n = 20 for ccndx−/−/hes2+. M–P Anti-Emx2 immunostaining (yellow) in myo6b:H2B-scarlet (magenta) expressing ccndx+/+ or ccndx−/− neuromasts with or without ccndx:hes2.2 expression. Q Quantification of Emx2+ hair cells per neuromast in ccndx+/+ or ccndx−/− mutants with or without ccndx:hes2.2 expression. Data are presented as mean values  + /− 95% confidence intervals. ****p < 0.0001, Two-way Anova (genotype, condition) with Šidák multiple comparisons test. n = 20 for ccndx+/+/hes2-, n = 16 for ccndx+/+/hes2+, n = 24 for ccndx−/−/hes2- and n = 20 for ccndx−/−/hes2+. R Quantification of hair cell number in in ccndx+/+ or ccndx−/−mutants with or without ccndx:hes2.2 expression. Data are presented as mean values  + /− 95% confidence intervals. **p = 0.0024, ***p = 0.001, Two-way Anova (genotype, condition) with Šidák multiple comparisons test. n = 20 for ccndx+/+/hes2-, n = 16 for ccndx+/+/hes2+, n = 24 for ccndx−/−/hes2- and n = 20 for ccndx−/−/hes2+.