FIGURE

Figure 2

ID

ZDB-FIG-250714-89

Publication

Wu et al., 2025 - FOXK2 in skeletal muscle development: a new pathogenic gene for congenital myopathy with ptosis

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Figure 2

foxk2 knockdown impaired skeletal muscle development of zebrafish.

(A) Structure of the zebrafish foxk2 gene. The zebrafish foxk2 gene was targeted using specific MOs to inhibit transcription at the start codon (ATG-MO) and proper splicing of exon 3 (I2E3-MO). (B) Quantitative of foxk2 expression level by qPCR. Larvae were collected at 72 hpf (n = 3 repeats). P value: ****P < 0.0001. (C) Percentage of larvae exhibiting defect or mortality. The control-MO group included 379 larvae, the ATG-MO group included 296 larvae, and the I2E3-MO group included 375 larvae. (D) Survival rate of larvae over a 72-hpf time course in control-MO, ATG-MO, and I2E3-MO groups. (E) Gross morphology of Tg(-1.9mylpfa:EGFP) larvae at 72-hpf, along with the quantification of curvature angle of larvae (n = 10 larvae for each group). The angle formed between the blue arrows indicates the degree of body curvature in the larvae. Scale bars: 500 µm. P value: ****P < 0.0001. (F) Body muscle morphology of Tg(-1.9mylpfa:EGFP) larvae at 72-hpf. Blue and yellow arrows denote normal and defective somites, respectively. The white asterisk indicates an abnormal trunk orientation. The inset in the upper right corner shows a magnified view of the boxed area (scale bars: 10 µm for the inset, 50 µm for the full image). (G) Cranial facial muscles of Tg(-1.9mylpfa:EGFP) larvae at 72-hpf. The inset in the upper right corner shows a magnified view of the boxed area (scale bars: 40 µm for the inset, 200 µm for the full image). (H–K) Statistical analysis of (H) movement distance (P value: ****P < 0.0001), (I) velocity (****P < 0.0001), (J) maximum acceleration (****P < 0.0001) and (K) mobility (percentage of actual moving distance to total distance) (***P = 0.0001; *P = 0.0231) in larvae at 5-dpf (n = 10 larvae for each froup). Data were analyzed by Student’s t test. All error bars indicate the mean ± standard deviation. MO morpholino, hpf hours post fertilization, qPCR quantitative real-time polymerase chain reaction, dpf days post fertilization. Source data are available online for this figure.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.