Fig. 5

Effect of CCZ on PP-IX levels and liver injury in DDC-fed mice. (A) Prominent liver-to-body weight ratio (LBW) was observed in mice fed with DDC-containing diet for 5 days (female DDC: 7.2 ± 0.4%; female normal diet [ND]: 4.7 ± 0.6%; male DDC: 8.7 ± 0.9%; male ND: 5.0 ± 0.1%). CCZ further increases the liver-to-body weight ratio in the DDC-fed female mice (female DDC + CCZ: 9.0 ± 0.8%). Data are shown as mean ± SD (n = 2–10 mice per group, N = 2). Statistical analysis was performed using the 1-way ANOVA test. ∗∗∗∗P < .0001; ∗∗P < .01. (B) Representative H&E liver sections and quantification of PP-IX deposit area in the livers of DDC-fed mice treated with PBS or CCZ. Arrows indicate PP-IX deposits. ∗∗∗ (P < .001) when comparing PBS with CCZ in females; ### (P < .001) when comparing PBS with CCZ in male mice. (C) PP-IX levels in the liver and stool isolated from the rectum. (D) Quantification of total PP-IX deposits in livers isolated from DDC-fed (5 days) mice. (E) Quantification of small-, medium-, and large-sized PP-IX deposits in the livers of DDC-fed mice. (F) Plasma ALT and ALP levels. Data are shown as mean ± SD (n = 5–10 mice per group, N = 2). Statistical analysis was performed using the 1-way ANOVA test. ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; ∗P < .05.