Fig. 4

Panx2 ablation regulates vision processes and structural components of the lens in larvae. a) A DESeq2 analysis of RNA-seq data from Panx2+/+ and Panx2Δ11 6 dpf zebrafish larvae identified 5355 differentially expressed genes with an adjusted P-value <0.05 b) Analysis of differentially expressed genes using GOSeq (v1.56.0). The Gene Ontology Classifier Biological Process (BP) showed significant downregulation of several vision-related categories. c,d) The most significantly down-regulated biological processes included Sensory Perception of Light Stimulus (GO:0050953) and Sensory Perception (GO:0007600). e) The top up-regulated BP was Humoral Immune Response (GO:0006959). f) The most significantly down-regulated Molecular Function (MF) was the Structural Component of the Lens (GO:0005212). g) STRING analysis of 61 regulated genes annotated as Sensory Perception of Light Stimulus (GO:0050953) and 17 regulated genes annotated as Humoral Immune Response (GO:0006959). K-means clustering identified two clusters which were either retina- or lens-specific. Cluster I genes are implicated in the phototransduction processes of the retina. Cluster II represents lens-specific proteins belonging to the alpha- or beta/gamma supergene families. A third group represents the humoral immune response. See also Supplementary Tables 2–5.