FIGURE

Fig. 1

ID

ZDB-FIG-250505-60

Publication

Shvartsburd et al., 2025 - Corticotropin-releasing hormone receptor 1 mediates the enhanced locomotor activity and metabolic demands to an acute thermal stress in adult zebrafish

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Fig. 1

Lack of CRHR1 affects the thermal stress-mediated cortisol response but not the cellular heat shock response. (A) Whole-body cortisol concentration of WT and crhr1−/− zebrafish sampled from the housing system at 28°C or after 90 min exposure to the intermittent flow respirometer at 33°C using an in-house cortisol ELISA. (B) Transcript abundance of hsp70, (C) hsp90.1, and (D) hsp90.2 transcript abundance, measured from epaxial muscle of WT and crhr1−/− zebrafish exposed to a novel tank environment containing 28°C or 33°C water for 1 h. Transcripts were normalized to β-Actin expression and WT 28°C fish were standardized to 1 using the 2−ΔΔCt method. (A) Different letters denote significant difference (Two-way ANOVA, Holm–Sidak post hoc test, p < 0.05). (B–D) Asterisks denote significant difference (Two-way ANOVA, Holm–Sidak post hoc test, p < 0.05). Crhr1, corticotropin-releasing hormone receptor 1; ELISA, enzyme-linked immunosorbent assay; hsp, heat shock protein; KO, crhr1-/-; WT, wildtype.

Expression Data

Genes:	hsp70.1 hsp90aa1.1 hsp90aa1.2
Fish:	WT crhr1^ca403/ca403
Condition:	heat shock
Anatomical Term:	myotome dorsal region
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT crhr1^ca403/ca403
Condition:	heat shock
Observed In:	myotome dorsal region whole organism cortisol
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neuroendocrinol.