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Fig. 1 Lack of CRHR1 affects the thermal stress-mediated cortisol response but not the cellular heat shock response. (A) Whole-body cortisol concentration of WT and crhr1−/− zebrafish sampled from the housing system at 28°C or after 90 min exposure to the intermittent flow respirometer at 33°C using an in-house cortisol ELISA. (B) Transcript abundance of hsp70, (C) hsp90.1, and (D) hsp90.2 transcript abundance, measured from epaxial muscle of WT and crhr1−/− zebrafish exposed to a novel tank environment containing 28°C or 33°C water for 1 h. Transcripts were normalized to β-Actin expression and WT 28°C fish were standardized to 1 using the 2−ΔΔCt method. (A) Different letters denote significant difference (Two-way ANOVA, Holm–Sidak post hoc test, p < 0.05). (B–D) Asterisks denote significant difference (Two-way ANOVA, Holm–Sidak post hoc test, p < 0.05). Crhr1, corticotropin-releasing hormone receptor 1; ELISA, enzyme-linked immunosorbent assay; hsp, heat shock protein; KO, crhr1-/-; WT, wildtype.