Fig. Ext Data 1

We introduce two designs for a multiscale microscope with dual-sided illumination (gray), a remote focusing unit (light gray) and detection with two resolutions (orange): a one design operating with cylindrical lenses and using a motorized slit, and b one design using Powell lenses, where no motorized slit is required (Supplementary Note 1). The microscope hardware comfortably accommodated several samples in one experiment, mounted in low melting agarose within fluorinated ethylene propylene (FEP) tubes. Abbreviations are as follows: 4 different lasers (LS1-LS4), dichroic beamsplitters (DC), 4x telescope (T1, f1?=?50?mm, f2?=?200?mm), pinhole (PI), halfwave plate (?1/2), polarized beamsplitter cube (PBS), motorized flip mirror (FM), two cylindrical lenses (CT, f1?=?25?mm, 100?mm), vertical slit (VS), resonant galvanometer (RM), low-resolution cylindrical lens (Cl, f?=?200?mm), tube lens (TLi, f?=?100?mm), illumination objective (IL, NA 0.4), telescope (T2, 5x), motorized vertical slit (mVS), high-resolution cylindrical lens (Ch, f?=?50?mm), tube lens (Trm, f?=?200?mm), remote focusing objective (RF), a quarterwave plate (QWP), telescope (T3, f1?=?200?mm, f2?=?75?mm), 10° Powell lens (P), f?=?30?mm lens (L1), f?=?60?mm lens (L2), f?=?400?mm lens (L3), voice coil with a mirror (VCM), LED illumination (LED), 20x NA1.0 detection objective (DL), filter wheel (FW), 100?mm tube lens (TL1), 500?mm tube lens (TL2).