Fig. Ext Data 1

We introduce two designs for a multiscale microscope with dual-sided illumination (gray), a remote focusing unit (light gray) and detection with two resolutions (orange): a one design operating with cylindrical lenses and using a motorized slit, and b one design using Powell lenses, where no motorized slit is required (Supplementary Note 1). The microscope hardware comfortably accommodated several samples in one experiment, mounted in low melting agarose within fluorinated ethylene propylene (FEP) tubes. Abbreviations are as follows: 4 different lasers (LS1-LS4), dichroic beamsplitters (DC), 4x telescope (T1, f1 = 50 mm, f2 = 200 mm), pinhole (PI), halfwave plate (λ1/2), polarized beamsplitter cube (PBS), motorized flip mirror (FM), two cylindrical lenses (CT, f1 = 25 mm, 100 mm), vertical slit (VS), resonant galvanometer (RM), low-resolution cylindrical lens (Cl, f = 200 mm), tube lens (TLi, f = 100 mm), illumination objective (IL, NA 0.4), telescope (T2, 5x), motorized vertical slit (mVS), high-resolution cylindrical lens (Ch, f = 50 mm), tube lens (Trm, f = 200 mm), remote focusing objective (RF), a quarterwave plate (QWP), telescope (T3, f1 = 200 mm, f2 = 75 mm), 10° Powell lens (P), f = 30 mm lens (L1), f = 60 mm lens (L2), f = 400 mm lens (L3), voice coil with a mirror (VCM), LED illumination (LED), 20x NA1.0 detection objective (DL), filter wheel (FW), 100 mm tube lens (TL1), 500 mm tube lens (TL2).