Fig. 2

a, Multiscale imaging of a SUM159 breast cancer cell spheroid embedded into a collagen matrix (Supplementary Videos 1 and 2) with the low-resolution modality shown on top and the high-resolution modality on the bottom. The spheroids consisted of a 1:1 mixture of cells expressing the actin marker Lifeact-GFP (cyan) and Lifeact-mCherry (magenta). Boxed region on top indicates the location of the high-resolution region on the bottom. The white arrowhead points at a cell division at the invasive front. b, Multiscale imaging of zebrafish gastrulation with cells expressing the histone marker Tg(h2afva:h2afva-GFP), starting at around 6?h after fertilization (Supplementary Videos 3 and 4). Low-resolution imaging (on top) captured the entire zebrafish embryo (color scale, depth of data in 3D volume from 0 to 450??m), while the high-resolution imaging (bottom) enabled near-simultaneous imaging of cell division (yellow arrowheads), including sister chromatid separations. c, Multiscale imaging of human breast cancer cells MDA-MB-231 expressing F-tractin-EGFP (left, magenta; right, grayscale) in situ in a larval zebrafish xenograft model (Supplementary Videos 5 and 6). The cancer cells were xenografted at 2.25 days post-fertilization into a zebrafish larvae expressing the vascular marker Tg(kdrl:Hsa.HRAS-mCherry) (cyan). Cell-spreading patterns as imaged in the low-resolution mode are shown on the left, and a 3D rendering of the high-resolution data is shown on the right. The white arrowhead points at a network of protrusions. Scale bars, 150??m (top), 20??m (bottom) (a); 200??m (top), 30??m (bottom) (b); 300??m (left); 40??m (right) (c)