FIGURE

Fig. 7

ID

ZDB-FIG-250221-45

Publication

Counil et al., 2025 - Brain penetration of peripheral extracellular vesicles from Alzheimer's patients and induction of microglia activation

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Fig. 7

Internalization of pEVs by endothelial cells in Tg(flk1:EGFP) zebrafish larvae at 2 and 3 dpf and brain homing. 4.5 nL of PBS or pEVs from either HeC or mAD patients (1 1010 pEVs/mL) were microinjected into the blood circulation of 2 days post-fertilization larvae, and the biodistribution was followed 1 and 24 hpi by in vivo confocal microscopy. (a) Live confocal images (maximum projection) at 1 and 24 hpi of the head of zebrafish larvae (20×) after injection of either PBS or pEVs (in red, indicated by arrows). Endothelial cells are in green. (b)–(c) Three-dimensional reconstruction on Imaris software of the head of the zebrafish in control (CTL) condition or 1 and 24 hpi of PKH-pEVs. Endothelial cells are in green, PKH-pEVs from mAD patients are in red, and colocalization areas are in white. (d) The percentage of internalization of PKH-pEVs by endothelial cells was quantified by using Imaris software and analysed by ordinary one-way ANOVA followed by Tukey's multiple comparisons test. Prior statistical analysis, the normality of each data set was evaluated with n = 7 per group.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Extracell Biol