FIGURE

Fig. 5

ID

ZDB-FIG-241211-5

Publication

Pelisenco et al., 2024 - miR-23b-3p, miR-126-3p and GAS5 delivered by extracellular vesicles inhibit breast cancer xenografts in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

EVs-based ncRNAs delivery to target breast cancer cells. Representative fluorescent microscopy images showing the uptake of MDA-MB-453 and MCF-7 derived extracellular EVs labeled in red by A MDA-MB-231 and B MDA-MB-453 recipient BC cells at 24 h post-treatment. Scale bars correspond to 30 μm for 63x magnification, and the fluorescent dyes used were DAPI (blue) for nuclei and CM-DiI (red) for EVs. The expression levels of cellular miR-23b-3p, miR-126-3p, and GAS5 were determined in terms of copies/µL using ddPCR technology. C Treatment with EVsorafenib derived from MDA-MB-453 resulted in an increased expression level of the three selected ncRNAs in MDA-MB-231 target cells. D Similarly, MDA-MB-453 target cells treated with EVsorafenib released by MCF-7 showed the same outcome. The histograms represent mean value of two replicates for each condition; bars, SD. *p < 0.05, **p < 0.01, ****p < 0.0001 in one-way ANOVA followed by Tukey’s test. Results are representative of two independent experiments

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Commun. Signal.