Fig. 7

Prolonged NF-kB resolution phase enhances the proliferative capabilities of nascent HSPCs.A WISH for the HE markers cmyb and gata2b in 44hpf AB embryos treated with DMSO (left), or 5 µM CAPE (right) from 28hpf. Red dashed boxes indicate the CHT region. Numbers in panels represent larvae with indicated phenotype. The experiment was repeated three times independently with similar results. B, C Representative confocal maximum projections of the DA (B), or CHT (C), from 52hpf cd41:eGFP+; kdrl:mCherry+ embryos incubated with DMSO or 5 µM CAPE from 28hpf. White arrowheads indicate cd41+ cells. Experiments were repeated three times independently with similar results. D Flow cytometric quantifications of total cd41+ HSPCs from pooled 52hpf cd41:eGFP+ embryos incubated with DMSO or 5 µM CAPE from 28hpf. Each dot represents the total cd41+ numbers within four pooled embryos. Black horizontal lines indicate mean ± SD. Data were analyzed using an unpaired two-tailed T-test. Source data are provided as a Source Data file.