FIGURE

Fig. 3

ID
ZDB-FIG-240715-81
Publication
Roisman-Geller et al., 2024 - Combined Pituitary Hormone Deficiency in lhx4-Knockout Zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

Whole-mount ISH analysis of pituitary hormone-encoding transcripts in 7 dpf larvae. (A,C–E) Transcript levels measured by the integrated densities of whole-mount-ISH-staining signals of tshb ((A); N = 10 homozygotes and 10 WTs), gh ((C); N = 11 homozygotes and 10 WTs), pomca ((D); N = 9 homozygotes and 4 WTs) and prl ((E); N = 9 homozygotes and 9 WTs) in 7 dpf lhx4 mutants and their WT siblings from heterozygous intercross. Homozygous lhx4 mutants expressed lower amounts of tshb compared with their WT siblings ((A); ** p < 0.01, Mann–Whitney test), while the expressions of gh, pomca and prl remained unaltered (C–E). Error bars indicate s.e.m. (B) Representative samples (heads of 7 dpf larvae, dorsal view) of WT sibling (left) and homozygous lhx4 mutant (right), analyzed by whole-mount ISH using the tshb probe. Tshb ISH signal (denoted by arrows) is considerably reduced in the pituitary of homozygous lhx4 mutant at 7 dpf as compared with its WT sibling. Scale bar = 100 µm.

Expression Data
Genes:
Fish:
Anatomical Term:
Stage: Days 7-13

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage: Days 7-13

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.