ptf1a and neurog1 are required for the development of GABAergic PCs and INs. (A-AF) Expression of parvalbumin 7 (Pvalb7, A-D), Pax2 (E-H), foxp1b (I-L), foxp4 (M-P), skor1b (Q-T), skor2 (U-X), lhx1a (Y-AB) and rorb (AC-AF) in the cerebellum of 5 dpf WT, neurog1 mutant, ptf1a mutant and ptf1a;neurog1 double-mutant larvae, revealed by immunostaining with anti-Pvalb7 (A-D) and anti-Pax2 (E-H) antibodies, or by in situ hybridization (I-AF). Dorsal views with anterior to the left. The cerebellum region (Ce) is surrounded by a dotted line (E-H). Pvalb7, foxp1b/4, skor1b/2, lhx1a, and rorb were expressed in PCs (expression of PC genes in the cerebellum is indicated by arrowheads). Pax2 is a marker of GABAergic INs. The number of examined larvae and larvae showing each expression pattern is given in Table 1. Scale bars: 50 μm (in A, for A-H); 100 μm (in I, for I-AF). (AG,AH) Number of Pvalb7+ PCs and Pax2+ INs in the cerebellum of 5 dpf WT, neurog1, ptf1a and ptf1a;neurog1 mutant larvae. **P<0.01, ***P<0.001, ****P<0.0001 (ANOVA with Tukey's multiple comparison test). Data are shown as mean±s.e.m. with individual values indicated. n=3 for each genotype.
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