FIGURE

Figure 3

ID

ZDB-FIG-240429-92

Publication

Jiang et al., 2024 - A Mechanistic Study of the Osteogenic Effect of Arecoline in an Osteoporosis Model: Inhibition of Iron Overload-Induced Osteogenesis by Promoting Heme Oxygenase-1 Expression

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Figure 3

ACL promoted the osteogenic differentiation of MC3T3-E1 cells under FAC treatment. (A) Cell viability of MC3T3-E1 cells treated with different concentrations of FAC (0–400 µmol/L) with or without ACL (5 or 10 mg/L) for 24 h. * p < 0.05 versus control group. (B,C) Alizarin red staining and Alkaline phosphatase staining of MC3T3-E1 cells treated with 100 μmol/L FAC and exposed to 5 or 10 mg/L ACL for 1 or 2 weeks. (D,E) Degree of mineralization and Alkaline phosphatase activity were quantified, * means p < 0.05 between two indicated groups (n = 6). (F–H) Quantitative analysis of mRNA expression levels of runt-related transcription factor 2 (Runx2), osteocalcin (OCN) and osteopontin (OPN) in MC3T3-E1 cells cultured in 100 μmol/L FAC with 5 or 10 mg/L ACL for 3 days. The control group was set to 1.0, * means p < 0.05 between two indicated groups (n = 6). Data are displayed as mean ± SD.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Antioxidants (Basel)