FIGURE

Figure 5

ID

ZDB-FIG-240429-87

Publication

Jiang et al., 2024 - A Mechanistic Study of the Osteogenic Effect of Arecoline in an Osteoporosis Model: Inhibition of Iron Overload-Induced Osteogenesis by Promoting Heme Oxygenase-1 Expression

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Figure 5

Ferroptosis was inhibited by ACL treatment under FAC exposure. (A,B) ROS production of zebrafish embryos (5 dpf) from control, FAC and FAC + (0.25 or 0.5 mg/L) ACL groups was detected and quantified by staining with DCF-DA, * means p < 0.05 between two indicated groups (n = 10). (C) Mitochondria ultrastructure of zebrafish embryo sections from control, FAC and FAC + ACL groups was imaged by transmission electron microscopy. Red arrows indicate damaged mitochondrial, including mitochondrial swelling, cristae lysis, and ruptured outer membrane. (D–F) Iron, GSH and MDA levels were determined in whole zebrafish embryos (5 dpf) from control, FAC, FAC + ACL and FAC + Fer1 groups, the concentrations used in the experiments were as follows: FAC (200 μg/mL), ACL (0.5 mg/L), and Fer1 (1 μM), data were displayed as mean ± SD, * means p < 0.05 between two indicated groups (n = 10).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Conditions:	chemical treatment by environment: arecoline chemical treatment by environment: arecoline chemical treatment by environment: ferric ammonium citrate chemical treatment by environment: ferric ammonium citrate
Observed In:	whole organism ferroptosis whole organism glutathione whole organism iron cation whole organism malonaldehyde whole organism mitochondrial crista whole organism mitochondrial outer membrane whole organism mitochondrion whole organism reactive oxygen species
Stage:	Day 5

Phenotype Detail

Acknowledgments

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