Figure 5

Figure 5

Ferroptosis was inhibited by ACL treatment under FAC exposure. (A,B) ROS production of zebrafish embryos (5 dpf) from control, FAC and FAC + (0.25 or 0.5 mg/L) ACL groups was detected and quantified by staining with DCF-DA, * means p < 0.05 between two indicated groups (n = 10). (C) Mitochondria ultrastructure of zebrafish embryo sections from control, FAC and FAC + ACL groups was imaged by transmission electron microscopy. Red arrows indicate damaged mitochondrial, including mitochondrial swelling, cristae lysis, and ruptured outer membrane. (D–F) Iron, GSH and MDA levels were determined in whole zebrafish embryos (5 dpf) from control, FAC, FAC + ACL and FAC + Fer1 groups, the concentrations used in the experiments were as follows: FAC (200 μg/mL), ACL (0.5 mg/L), and Fer1 (1 μM), data were displayed as mean ± SD, * means p < 0.05 between two indicated groups (n = 10).