FIGURE

Fig. 3.

ID

ZDB-FIG-240129-123

Publication

Koosha et al., 2023 - Proteoglycan inhibition of canonical BMP-dependent cartilage maturation delays endochondral ossification

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3.

Smad-mediated BMP signalling is increased in fam20b^−/− chondrocytes. (A-D′) Fluorescence immunostaining showed no clear differences between wild types (A,C,C′) and fam20b mutants (B,D,D′) in the levels of p-Smad1/5/9 in nuclei and non-nuclear regions of condensing ceratohyal mesenchyme at 48 hpf. (E-H′) p-Smad1/5/9 immunoreactivity appeared higher in non-nuclear regions of fam20b^−/− ceratohyal chondrocytes (F,H,H′) at 72 hpf, compared with wild types (E,G,G′). (I-J′) p-Smad1/5/9 immunoreactivity appeared increased in both the nuclei and non-nuclear regions of fam20b^−/− chondrocytes (J,J′), compared with wild types (I,I′), at 84 hpf. (K) Quantitative image analyses (n=3 for each group) demonstrated significant increases in p-Smad1/5/9 levels of fam20b^−/− chondrocytes (*P<0.05, one-way ANOVA and paired Student's t-test). Scale bars: 50 μm. a.u., arbitrary units; ch, ceratohyal; oc, oral cavity; wt, wild type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development