FIGURE

Fig. 2.

ID

ZDB-FIG-240129-122

Publication

Koosha et al., 2023 - Proteoglycan inhibition of canonical BMP-dependent cartilage maturation delays endochondral ossification

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2.

Histological and molecular markers show similar timing of early cartilage formation between wild types and fam20b mutants. (A-B′) Lack of Safranin O staining demonstrated that developing ceratohyal cells of wild-type and fam20b mutant zebrafish did not secrete cartilage PGs at 48 hpf. (C-F) Cells in the mesenchymal condensation of both wild types (C,C′,E) and fam20b mutants (D,D′,F) were specified to form cartilage, as evidenced by col2a1a (C-D′) and sox9a (E,F) expression. (G-H′) The spacing between chondrocytes suggested that cartilage PG secretion had occurred in ceratohyal chondrocytes of both wild-type and fam20b mutant zebrafish at 72 hpf, but light Safranin O staining was only observed in wild types. Scale bars: 50 μm. ch, ceratohyal; oc, oral cavity; SafO, Safranin O.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development