FIGURE

Fig. 11.

ID

ZDB-FIG-240129-133

Publication

Koosha et al., 2023 - Proteoglycan inhibition of canonical BMP-dependent cartilage maturation delays endochondral ossification

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Fig. 11.

Inhibition of BMP signalling by DMH1 or by heat shocking dnBMPR zebrafish embryos rescues the increased BMP signalling in fam20b^−/− chondrocytes. (A-D′) Compared with DMSO-treated (A,A′) or heat-shocked (C,C′) fam20b^−/− chondrocytes, levels of p-Smad1/5/9 at 72 hpf appeared decreased in DMH1-treated fam20b^−/− (i.e. after 24 h of treatment; B,B′) or heat-shocked dnBMPR; fam20b^−/− (i.e. 2 days after 20-min heat shock; D,D′) chondrocytes. (E) Quantitative image analyses (n=3 for each group) revealed significant decreases in p-Smad1/5/9 levels in both nuclei and non-nuclear regions of DMH1-treated or heat-shocked fam20b^−/− chondrocytes, compared with each control group (*P<0.05, one-way ANOVA and paired Student's t-test). (F-G′) Compared with DMSO-treated fam20b^−/− chondrocytes (F,F′), levels of p-p38 at 72 hpf (i.e. after 24 h of treatment) appeared decreased in DMH1-treated fam20b^−/− chondrocytes (G,G′). (H) Quantitative image analyses (n=3 for each group) revealed significant decreases in p-p38 levels in nuclei of DMH1-treated fam20b^−/− chondrocytes (*P<0.05, one-way ANOVA and paired Student's t-test). Scale bars: 50 μm. a.u., arbitrary units; HS, heat-shocked.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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