FIGURE

Fig. 10.

ID

ZDB-FIG-240129-132

Publication

Koosha et al., 2023 - Proteoglycan inhibition of canonical BMP-dependent cartilage maturation delays endochondral ossification

Other Figures

All Figure Page

Back to All Figure Page

Fig. 10.

Inhibition of BMP signalling by heat shocking dnBMPR zebrafish embryos reduces cartilage maturation gene expression and perichondral bone formation. (A-H) Compared with chondrocytes in non-heat-shocked wild-type (A,B) and dnBMPR (C,D) embryos and heat-shocked wild-type (E,F) embryos, heat-shocked dnBMPR (G,H) chondrocytes had decreased expression of the chondrocyte maturation genes ihha and col10a1a at 4 dpf (i.e. 3 days after heat shock). These are representative images from at least 12 samples for each group (at least six samples each from two clutches). (I-L″) Compared with control groups (I-K″), perichondral bone appeared to decrease in heat-shocked dnBMPR embryos (L-L″) at 7 dpf (i.e. 6 days after heat shock). (M) Quantitation of 100 embryos (20 embryos each from five clutches) for each experimental group confirmed a significant decrease in perichondral bone in heat-shocked dnBMPR embryos (*P<0.05, one-way ANOVA and paired Student's t-test). Scale bars: 50 μm (A-H); 200 μm (I-L″). A, anterior; AB/AR, Alcian Blue/Alizarin Red; Ch, ceratohyal cartilage; Chb, ceratohyal bone; Hm, hyomandibular bone; Hs, hyosymplectic cartilage; HS, heat-shocked; L, lateral; wt, wild type.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development