FIGURE

Figure 7

ID

ZDB-FIG-231116-37

Publication

Taler et al., 2023 - Identification of Small Molecules for Prevention of Lens Epithelium-Derived Cataract Using Zebrafish

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Figure 7

mTOR signaling contributes to cell mass growth. (A,B) Confocal single-plane sections from eyes of 3 dpf plod3^vu222 larvae labeled for pS6K (green) and with 5E11 antibody that marks nuclei of ALE cells as well as a subset of amacrine cells (purple). pS6K staining in the mutant is increased in the developing mass and reduced in the ciliary marginal zones (marked by asterisks in (A)). White rectangles mark the ALE region. Scale bar is 50 µm. (C,D) Histological sections from eyes of 4 dpf plod3^vu222 larvae treated from 26 hpf until ~96 hpf with vehicle (C) or rapamycin (D). White lines mark the periphery of cellular masses. Scale bar is 20 µm. L, lens.

Expression Data

Antibodies:	Ab-5E11 Ab1-rps6
Fish:	WT plod3^vu222/vu222
Anatomical Terms:	amacrine cell lens epithelium anterior side lens epithelium nucleus
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	plod3^vu222/vu222
Condition:	chemical treatment by environment: sirolimus
Observed In:	lens epithelium
Stage:	Day 4

Phenotype Detail

Acknowledgments

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