FIGURE

Figure 3

ID

ZDB-FIG-231115-17

Publication

Li et al., 2023 - Stanniocalcin 1a regulates organismal calcium balance and survival by suppressing Trpv6 expression and inhibiting IGF signaling in zebrafish

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Figure 3

Stc1a and Trpv6 suppress NaR cell proliferation via the same IGF signaling pathway. (A) Inhibition of Trpv6 abolishes the elevated NaR cell proliferation in stc1a^-/- larvae. Larvae (3 dpf) of the indicated genotypes were treated with DMSO or 10 μg/L CdCl₂ for 2 days. GFP-labeled NaR cells were quantified and shown. n = 4~19 fish/group. *, P < 0.05. ns, not statistically significant. (B, C)stc1a^-/-; trpv6^-/- double mutants phenocopy trpv6^-/- fish. Progeny of stc1a^+/-; trpv6^+/- in the Tg(igfbp5a:GFP) background were raised in E3 medium. At 5dpf, NaR cells were quantified and shown. These larvae were genotyped individually Representative images are shown in (B) and quantified data in (C). n = 4~19 larvae/group. Scale bar = 0.2 mm. (D, E) Progenies of stc1a^+/-; trpv6^+/- intercrosses were raised in E3 medium. They were subjected to whole mount immunohistochemistry using an anti-phospho-Akt antibody. Phospho-Akt positive cells in the yolk sac region were quantified. The larvae were genotyped individually afterwards. Representative images are shown in (D) and quantified data in (E). n = 5~14 larvae/group. Scale bar = 0.2 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	trpv6^mi604/mi604 stc1l^mi610/mi610; mi602Tg trpv6^mi604/mi604; stc1l^mi610/mi610 trpv6^mi604/mi604; stc1l^mi610/mi610; mi602Tg
Condition:	chemical treatment by environment: cadmium dichloride
Observed In:	NaK ionocyte NaK ionocyte insulin-like growth factor receptor signaling pathway
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)