Figure 3

Figure 3

Stc1a and Trpv6 suppress NaR cell proliferation via the same IGF signaling pathway. (A) Inhibition of Trpv6 abolishes the elevated NaR cell proliferation in stc1a^-/- larvae. Larvae (3 dpf) of the indicated genotypes were treated with DMSO or 10 μg/L CdCl₂ for 2 days. GFP-labeled NaR cells were quantified and shown. n = 4~19 fish/group. *, P < 0.05. ns, not statistically significant. (B, C)stc1a^-/-; trpv6^-/- double mutants phenocopy trpv6^-/- fish. Progeny of stc1a^+/-; trpv6^+/- in the Tg(igfbp5a:GFP) background were raised in E3 medium. At 5dpf, NaR cells were quantified and shown. These larvae were genotyped individually Representative images are shown in (B) and quantified data in (C). n = 4~19 larvae/group. Scale bar = 0.2 mm. (D, E) Progenies of stc1a^+/-; trpv6^+/- intercrosses were raised in E3 medium. They were subjected to whole mount immunohistochemistry using an anti-phospho-Akt antibody. Phospho-Akt positive cells in the yolk sac region were quantified. The larvae were genotyped individually afterwards. Representative images are shown in (D) and quantified data in (E). n = 5~14 larvae/group. Scale bar = 0.2 mm.