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Aptamers rescue defective myelopoiesis in mouse and iPSC from DC patients. a Left graph; comparison of the composition of erythroid and myeloid colonies assayed by colony assays, using whole bone marrow cells from mice of the indicated phenotype. Middle and right graphs; composition of erythroids and myeloids assayed by colony assays, using whole bone marrow cells from mice of the indicated phenotype an incubated with the mouse T1000 aptamer. N = 7 for wt and Terc -/- mice and N = 5 for Tert -/- mice. b Induced-pluripotent stem cell (iPSC) workflow. c Composition of erythroids and myeloids assayed by colony assays, using embryonic bodies (EBs) derived from the indicated iPSC lines treated with different aptamers. d Quantification of the hemogenic progenitors (HEPs) population (CD31 + /CD34 + /CD45-) by flow cytometry at day 15 in EBs. e mRNA levels of SPI1 assayed by RT-qPCR in the different conditions. The expression is normalized to GAPDH and relative to control sample. a Data are average of at least 5 biologically independent samples (wt, n = 7; Tert-/-, n = 5; Terc-/-, n = 7). Bars represent mean ± SEM. Statistical analysis according to 2way ANOVA followed by Dunnett’s multiple comparison test (95% confidence interval). c Data are average of 3 biologically independent samples. Bars represent mean ± SEM. Statistical analysis according to 2way ANOVA followed by Dunnett’s multiple comparison test (95% confidence interval). d Data are average of 3 biologically independent samples represented by dots. Bars represent mean ± SEM for each group. n.s., not significant, p > 0.05 according to Kruskal-Wallis followed by Dunn’s multiple comparison test (95% confidence interval). e Data are average of 3 biologically independent samples represented by dots. Bars represent mean ± SEM. Statistical analysis according to Kruskal-Wallis followed by Dunn’s multiple comparison test (95% confidence interval). Source data are provided as a Source Data file.
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