FIGURE

Figure 5

ID

ZDB-FIG-230814-207

Publication

Suzuki et al., 2023 - Cooperative contributions of the klf1 and klf17 genes in zebrafish primitive erythropoiesis

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Figure 5

Differential expression of haematopoietic genes in the klf1-klf17 mutant. (A,C,E,G,I,K,M,O) Wild-type embryos with wild-type alleles of klf1 and klf17 at 24 hpf. (B,D,F,H,J,L,N,P) klf1-klf17 mutant at 24 hpf. Whole-mount in situ hybridization (WISH) with scl (A,B), c-myb (C,D), fech (E,F), alas2 (G,H), sptb (I,J), band3 (K,L), dmt1 (M,N) and mitoferrin (O,P). All pictures show lateral views, with the anterior to the left. Scale bar, 200 μm (A). Arrowheads indicate the position of the ICM. The expression of scl, c-myb, fech, sptb and dmt1 at 24 hpf was comparable between the wild-type and klf1-klf17 mutant. In contrast, the expression of alas2, band3 and mitoferrin was decreased in the klf1-klf17 mutant. Genotyping of individual embryos was performed by genomic PCR.

Expression Data

Genes:	alas2 fech myb slc4a1a slc11a2 slc25a37 sptb tal1
Fish:	WT klf1^uy19/uy19; klf17^uy21/uy21
Anatomical Term:	intermediate cell mass of mesoderm
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	klf1^uy19/uy19; klf17^uy21/uy21
Observed In:	intermediate cell mass of mesoderm
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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