ZFIN Image: Suzuki et al., 2023, Figure 5

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Figure 5

Differential expression of haematopoietic genes in the klf1-klf17 mutant. (A,C,E,G,I,K,M,O) Wild-type embryos with wild-type alleles of klf1 and klf17 at 24 hpf. (B,D,F,H,J,L,N,P) klf1-klf17 mutant at 24 hpf. Whole-mount in situ hybridization (WISH) with scl (A,B), c-myb (C,D), fech (E,F), alas2 (G,H), sptb (I,J), band3 (K,L), dmt1 (M,N) and mitoferrin (O,P). All pictures show lateral views, with the anterior to the left. Scale bar, 200 μm (A). Arrowheads indicate the position of the ICM. The expression of scl, c-myb, fech, sptb and dmt1 at 24 hpf was comparable between the wild-type and klf1-klf17 mutant. In contrast, the expression of alas2, band3 and mitoferrin was decreased in the klf1-klf17 mutant. Genotyping of individual embryos was performed by genomic PCR.

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Acknowledgments

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