FIGURE

Fig 6

ID

ZDB-FIG-230526-60

Publication

Aljiboury et al., 2023 - Rab8, Rab11, and Rab35 coordinate lumen and cilia formation during zebrafish left-right organizer development

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Fig 6

Rab11 and Rab35, but not Rab8, regulates KV lumen formation by mediating CFTR trafficking to the apical membrane.

(A) Optogenetic clustering of Rab11, Rab8, and Rab35 (magenta) in KV cells. Localization with CFTR-GFP (inverted gray) is shown. Scale bar, 20 μm. (B) Violin plots depicting percent of optogenetic clusters that colocalize with CFTR. n>9 embryos, **p<0.01, ****p<0.0001. (C) Optogenetic clustering of CIB1-mCherry-Rab11 and CIB1-mRuby-Rab35 (cyan). CIB1-mCherry-Rab11 clusters localization with Flag-Rab8 (magenta) or CIB1-mRuby-Rab35 clusters with GFP-Rab11 (magenta) shown. Scale bar, 7 μm. (D) Violin plot depicting percent of optogenetic clusters that colocalize with Rab8, Rab35, or Rab11. n>9 embryos, ****p<0.0001. Statistical results detailed in S1 Table.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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