Figure 7
- ID
- ZDB-FIG-230228-58
- Publication
- Nakamura et al., 2023 - Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord
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(A) The experimental system to analyze treadmill locomotion. Trajectories of fore- and hind-paw were tracked by DeepLabCut and kinematic parameters including step and stride lengths were analyzed by KinemaTracer. (B–F) Analyses of treadmill locomotion pre- and post-inactivation of hM4Di+ CSF-cNs by clozapine-N-oxide (CNO) treatment. Pkd2l1-Cre mice were intracerebroventricularly injected with adeno-associated virus (AAV)-Syn-DIO-hM4Di-mCherry to introduce hM4Di-mCherry. (B) Maximum treadmill speeds in a program increasing from 8.33 to 58.33 cm/s (5.0–35 m/min). (C–F) Circular phase plots representing phase values of the left fore- and hind-paw contacts between two right paw contacts and r (C), step frequency (D), stride lengths (E), and step lengths (F) of the left fore- and hindlimbs at a speed of 23.3 cm/s (14 m/min). CNO-treated groups are represented in blue. (G–K) Analyses of treadmill locomotion of AAV-Syn-DIO-hM4Di-mCherry-injected wild-type (WT) mice pre- and post-CNO treatment. Maximum treadmill speeds (G), circular phase plots (H), step frequency (I), stride lengths (J), and step lengths (K) of the left fore- and hindlimbs. (L, M) Average number of fault placements in the ladder walk test pre- and post-CNO treatment in AAV-Syn-DIO-hM4Di-mCherry-injected Pkd2l1-Cre (L) and WT mice (M). (N, O) The number of slips in five runs of the beam walk test pre- and post-CNO treatment in AAV-Syn-DIO-hM4Di-mCherry-injected Pkd2l1-Cre (N) and WT mice (O). Paired t-test (B,D–F, G, I–M), Wilcoxon matched-pairs signed rank test (N, O); *p<0.05, ** p<0.01; the mean ± standard error of the mean (SEM); n=7 (B–L, N), n=6 (M, O).
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