PUBLICATION
Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord
- Authors
- Nakamura, Y., Kurabe, M., Matsumoto, M., Sato, T., Miytashita, S., Hoshina, K., Kamiya, Y., Tainaka, K., Matsuzawa, H., Ohno, N., Ueno, M.
- ID
- ZDB-PUB-230223-57
- Date
- 2023
- Source
- eLIFE 12: (Journal)
- Registered Authors
- Keywords
- cerebrospinal fluid-contacting neuron, locomotion, mouse, neural circuit, neuroscience, spinal cord
- MeSH Terms
-
- Zebrafish*
- Receptors, Cell Surface
- Mice
- Locomotion*
- Neurons, Efferent
- Interneurons
- Animals
- Calcium Channels
- Motor Neurons
- Mammals
- PubMed
- 36805807 Full text @ Elife
Citation
Nakamura, Y., Kurabe, M., Matsumoto, M., Sato, T., Miytashita, S., Hoshina, K., Kamiya, Y., Tainaka, K., Matsuzawa, H., Ohno, N., Ueno, M. (2023) Cerebrospinal fluid-contacting neuron tracing reveals structural and functional connectivity for locomotion in the mouse spinal cord. eLIFE. 12:.
Abstract
Cerebrospinal fluid-contacting neurons (CSF-cNs) are enigmatic mechano- or chemosensory cells lying along the central canal of the spinal cord. Recent studies in zebrafish larvae and lampreys have shown that CSF-cNs control postures and movements via spinal connections. However, the structures, connectivity, and functions in mammals remain largely unknown. Here we developed a method to genetically target mouse CSF-cNs that highlighted structural connections and functions. We first found that intracerebroventricular injection of adeno-associated virus with a neuron-specific promoter and Pkd2l1-Cre mice specifically labeled CSF-cNs. Single-cell labeling of 71 CSF-cNs revealed rostral axon extensions of over 1800 μm in unmyelinated bundles in the ventral funiculus and terminated on CSF-cNs to form a recurrent circuitry, which was further determined by serial electron microscopy and electrophysiology. CSF-cNs were also found to connect with axial motor neurons and premotor interneurons around the central canal and within the axon bundles. Chemogenetic CSF-cNs inactivation reduced speed and step frequency during treadmill locomotion. Our data revealed the basic structures and connections of mouse CSF-cNs to control spinal motor circuits for proper locomotion. The versatile methods developed in this study will contribute to further understanding of CSF-cN functions in mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping