Expression of ngn1, Islet-1 and gfap is reduced in Syn III-MO injected embryos. (A) Representative images of ST-MO and Syn III-MO-injected zebrafish embryos hybridized with ngn1 at 16 hpf and 24 hpf. Abbreviations: mb: midbrain, hb, hindbrain; cg, cranial ganglia; t, telencephalon; d, diencephalon. 16 hpf scale bar, 200 ?m; 24 hpf scale bar, 500 ?m. (B,C) Quantification of ngn1 signal at 16 hpf (B) and 24 hpf (C) as mean intensity values in arbitrary units (A.U.). Syn III-MO-injected embryos showed a significant decrease in the mean intensity value when compared to control embryos (*** p < 0.001, unpaired Student?s t-test, n = 12 embryos per group). (D,E) Representative images showing 30 hpf embryos hybridized with Islet-1 (D) and gfap (E), scale bar = 200 ?m for all the panels. Abbreviations: d, diencephalon; f: forebrain; hb, hindbrain; hbn, hindbrain neurons; mhb, midbrain hindbrain boundary; mb, midbrain; on, optic nerve; t, telencephalon. (F,G) Quantification of Islet-1 (F) and gfap (G) signal expressed as mean intensity values. Syn III-MO-injected embryos exhibited a significant decrease of both Islet-1 ((F), *** p < 0.001 unpaired Student?s t-test) and gfap when compared to control embryos ((G), ** p < 0.01, unpaired Student?s t-test). n = 6?15 embryos per group).
