Fig. 2
- ID
- ZDB-FIG-220920-70
- Publication
- Tian et al., 2022 - WRN promotes bone development and growth by unwinding SHOX-G-quadruplexes via its helicase activity in Werner Syndrome
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- (all 9)
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Loss of wrn causes bone abnormalities in zebrafish.
a, b Representative WISH analysis of 3 independent experiments of wrn expression between WT and wrn?/? mutant zebrafish on both 7 dpf and 10 dpf. c, e, g, i Representative Masson?s trichrome staining of 3 independent experiments between WT and wrn?/? mutant zebrafish on 14 dpf (c WT; e wrn?/? mutants) and 40 dpf (g WT; i, wrn?/? mutants). Scale bar = 100 µm. Notochord sheath (nsh, red arrow indicated) and notochord vaculated cells (vc, red arrow indicated). Intercentral joint (ij, red encircled) and centrum (ct). d, h, f, j Representative FISH analysis of 3 independent experiments of wrn expression and BrdU staining between WT and wrn?/? mutant zebrafish on 14 dpf (d WT; f wrn?/? mutants) and 40 dpf (h WT; j wrn?/? mutants). k?n Representative WISH analysis of 3 independent experiments of chondrogenic markers (sox9a, col2a1a, col10a1a, and col1a1a) between WT and wrn?/? mutant zebrafish on 7 dpf. Scale bar = 50 µm. o?v Representative FISH analysis combined with BrdU staining of 3 independent experiments of chondrogenic markers (sox9a, col2a1a, and col10a1a) between WT and wrn?/? mutant zebrafish on 14 dpf (o, p WT; q, r wrn?/? mutants) and chondrogenic markers (col2a1a, col10a1a, and col1a1a) on 40 dpf (s, t WT; u, v wrn?/? mutants). Scale bar = 100 µm. |
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Stage Range: | Days 7-13 to Days 30-44 |
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Observed In: | (all 8) |
Stage Range: | Days 7-13 to Days 30-44 |