Fig. 4

Figure 4. Activity pattern of V0d interneuron subtypes during fictive locomotion in adult zebrafish

(A) Recordings of a slow V0d interneuron showing recruitment across all swimming frequencies. The image above each trace shows a ventral root recording.

(B) Plot of the instantaneous swim frequency (filled black circles) and membrane potential oscillation amplitude (filled red circles) for a slow V0d interneuron during a swim episode. Filled red circles denote swim cycles with recruitment.

(C) Slow V0d interneurons showed large oscillation amplitudes across swim frequencies (1–3 Hz: n = 34 slow V0d interneurons; 4–6 Hz: n = 17 slow V0d interneurons; ^∗p < 0.05; unpaired two-tailed Student’s t test).

(D) Recordings of an intermediate V0d interneuron showing recruitment at intermediate frequencies (4–6 Hz).

(E) Plot of the instantaneous swim frequency (filled black circles) and membrane potential oscillation amplitude (green circles) for an intermediate V0d interneuron during a swim episode. Filled green circles denote swim cycles with recruitment, and open green circles denote no recruitment.

(F) Intermediate V0d interneurons showed large oscillation amplitudes in the intermediate frequency range (1–3 Hz: n = 29 intermediate V0d interneurons; 4–6 Hz: n = 23 intermediate V0d interneurons; ^∗∗∗p < 0.0001; unpaired two-tailed Student’s t test).

(G) Recordings of a fast V0d interneuron showing no recruitment at slow or intermediate frequencies.

(H) Plot of the instantaneous swim frequency (filled black circles) and membrane potential oscillation amplitude (open blue circles) for a fast V0d interneuron during a swim episode. Open blue circles denote swim cycles with no recruitment.

(I) Fast V0d interneurons showed small oscillation amplitudes in the slow and intermediate frequency ranges (1–3 Hz: n = 18 fast V0d interneurons; 4–6 Hz: n = 15 fast V0d interneurons; unpaired two-tailed Student’s t test).

See also Figure S2.