Fig. 4

Lipid accumulation in RPE cells of WT and cyp4v7^−/−cyp4v8^−/− zebrafish. (A) Nile red staining of lipid droplets in retinal frozen sections of WT and cyp4v7^−/−cyp4v8^−/− zebrafish at 3, 7, 10, and 12 mpf. White arrows, lipid droplets. Scale bar: 20 µm. (B) Nile red staining of RPE flat mounts from WT and cyp4v7^−/−cyp4v8^−/− zebrafish at 3, 7, and 10 months after fertilization. White arrows, lipid droplets (bright red). Scale bar: 20 µm. Bar graphs show LD size distribution in cyp4v7^−/−cyp4v8^−/− zebrafish RPE flat mounts at 3, 7, and 10 months after fertilization. Diameter of LDs in cyp4v7^−/−cyp4v8^−/− zebrafish RPE cell was measured and represented by white (>3 μm), black (2-3 μm), red (1-2 μm), and blue (0-1 μm) (n = 88-103 RPE cells examined over three biologically independent experiments). (C) TEM images of retinal ultrathin sections from WT and cyp4v7^−/−cyp4v8^−/− zebrafish at seven months after fertilization. Lipid droplets are labeled with red text “LD”. Vacuoles are labeled with red text “V.”