Fig. 1

Effect of rig-I knockdown on the expression of myeloid-lineage and granulocyte marker genes

(A) General overview of primitive hematopoietic wave during zebrafish embryonic development. (B−E) Expression of myeloid precursor marker pu.1/spi1 in control MO (B and D) or rig-I morphant (C and E) embryos at the six-somite (dorsal view) stage and 24 hpf (24 h, lateral view). Anterior regions of 24-hpf embryos are shown in lateral view at higher magnification in the right panel. (F and G) Expression of macrophage marker lcp1/l-plastin in control MO (F) or rig-I morphant (G) embryos at 24 hpf (24 h, lateral view). (H−K) Expression of granulocyte marker mpx in embryos injected with control MO from lateral view (H) and dorsal view (J) or rig-I morphant embryos from lateral view (I) and dorsal view (K) at 48 hpf. Anterior regions of 48-hpf embryos injected with rig-I MO are shown in lateral and dorsal views at higher magnification in the right panel. (L and M) Detection of granules in granulocytes staining with SBB in control MO (L) or rig-I morphant (M) embryos at 36 hpf. (N−Q) Percent of embryos with phenotype of normal or decreased staining intensity in control or rig-I morphant embryos from (B)–(M) based on WISH analysis or SBB staining. The number of embryos used for statistics is shown in each figure. Images were captured under Olympus stereoscope (MVX10 MacroView; original magnification ×50). Each experiment was repeated three times (n = 3, mean ± SD, Student t test, **p < 0.01, ***p < 0.001).