Fig. 4

Effect of rig-I mRNA overexpression on the generation of hematopoietic precursors

(A−I) Expression of hematopoietic precursor marker scl/tal1 at the six-somite stage in embryos injected with control MO from dorsal view (A and B) and lateral view (C), rig-I MO from dorsal view (D and E) and lateral view (F), or rig-I MO and mRNA from dorsal view (G and H) and lateral view (I). (J−R) Expression of hematopoietic precursor marker scl/tal1 at the six-somite stage in embryos injected with control MO from dorsal view (J and K) and lateral view (L), rig-I MO from dorsal view (M and N) and lateral view (O), or rig-I MO and mRNA from dorsal view (P and Q) and lateral view (R). Images were captured under Olympus stereoscope (MVX10 MacroView; original magnification ×50). (S and T) Percent of embryos with phenotype of normal or decreased staining intensity in control or rig-I morphant embryos from (A)–(R) based on WISH analysis. The number of embryos used for statistics is shown in each figure. Each experiment was repeated three times (n = 3, mean ± SD, Student t test, **p < 0.01, ***p < 0.001; ns, not significant).