FIGURE

FIG 5

ID

ZDB-FIG-220305-38

Publication

Romartinez-Alonso et al., 2021 - Structure-guided approach to relieving transcriptional repression in Resistance to Thyroid Hormone α

Other Figures

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FIG 5

Characterization of P393GX LBD. (A) Corepressor binding assay of P393GX by fluorescence anisotropy showing saturating-binding curves and dissociation constant (Kd) of the P393GX binding to corepressor peptide in the absence and presence of T3. Error bars indicate mean ± SEM (n = 5). (B) Thermal stability (melting temperature) of P393GX in the apo form, with T3, with SMRT peptide, and with both T3 and SMRT peptide. (C) Purification of P393GX by GST and ion-exchange chromatography, illustrated via SDS-PAGE. (D) A 2F0-Fc electron density map contoured at 1.0 σ around the binding pocket of the protein, clearly showing T3 and the residues conforming the ligand binding pocket (structure deposited with PDB ID 7QDT).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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