Characterization of early onset of adipogenesis and energy balance in gMXs and hMXs larvae. (A) Left: whole-mount ORO staining of WT ± Dox and gMXs (1?3) ± Dox larvae at 24 dpf. Scale bar: 100 ?m. Right: the percentage of ORO-stained zebrafish larvae. (B) Left: whole-mount ORO staining of WT ± Dox and hMXs (1, 3, and 4) ± Dox larvae at 24 dpf. Scale bar: 125 ?m. Right: the percentage of ORO-stained zebrafish larvae. (C) Molecular analysis of gMX2 and hM3 larvae revealed the upregulation of lipogenic genes, ppar-?, C/EBP-?, srebp1, fasn, acaca, chrebp, acc1, and dgat2. (D) Molecular analysis of gMX2 and hM3 larvae revealed an upregulation of adipogenesis genes, c/ebp-?, c/ebp-?, c/ebp-?, ptip, klf4, zfp423, ezh2, and tcf7l1. (E) Response to gMX2 and hM3 larvae by the Alamar Blue assay. Data are conveyed as the relative difference in signal intensity in three discrete assays (mean ± SEM, n = 40), Statistically significant differences from the controls are indicated by * (p < 0.05), and ** (p < 0.01).
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