FIGURE

Fig. 6

ID

ZDB-FIG-211118-26

Publication

Laplace-Builhé et al., 2021 - NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration

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Fig. 6

<italic>Erbb2</italic> is expressed by all blastemal cells including macrophages, and particularly pro-inflammatory macrophages.

aTg(mpeg1:mCherry-F/tnfa:eGFP-F) larvae were amputated or not (control) at 3 dpf, and cells were dissociated at 6 or 24 hpA and sorted by FACS. Red, green, and black gates represent mCherry⁺, mCherry⁺eGFP⁺, and mCherry⁻eGFP⁻cell populations, respectively. Relative expression of b, enrg1, c, ferbb2, and d, gerbb3 in mCherry⁻eGFP⁻, mCherry⁺ and mCherry⁺eGFP⁺ cells at 6 hpA b, c, d and 24 hpA e, f, g was quantified by RT-PCR on separated cells using ef1a as reference gene. b, c, d Graphs represent the mean value ± SEM, n = 200-300 pooled larvae from 4 independent experiments; one-tailed Mann-Whitney test was performed, p = 0.0286, *p < 0.05. e, f, g Graphs represent the mean value ± SEM, n = 200–300 pooled larvae from 6 independent experiments; one-tailed Mann-Whitney test was performed, ep = 0.0143, fp = 0.0465, *p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.