PUBLICATION
NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration
- Authors
- Laplace-Builhé, B., Barthelaix, A., Assou, S., Bohaud, C., Pratlong, M., Severac, D., Tejedor, G., Luz-Crawford, P., Nguyen-Chi, M., Mathieu, M., Jorgensen, C., Djouad, F.
- ID
- ZDB-PUB-211105-1
- Date
- 2021
- Source
- Nature communications 12: 6336 (Journal)
- Registered Authors
- Djouad, Farida
- Keywords
- none
- Datasets
- GEO:GSE158851
- MeSH Terms
-
- Animal Fins/metabolism*
- Animals
- Cell Proliferation
- Forkhead Transcription Factors/metabolism
- Gene Expression Regulation, Developmental
- Genes, erbB/genetics*
- Larva
- Macrophages/metabolism*
- Neural Crest/metabolism*
- Neuregulin-1/genetics
- Neuregulin-1/metabolism*
- Regeneration/genetics
- Regeneration/physiology*
- Signal Transduction/genetics
- Signal Transduction/physiology*
- Stem Cells
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- PubMed
- 34732706 Full text @ Nat. Commun.
Citation
Laplace-Builhé, B., Barthelaix, A., Assou, S., Bohaud, C., Pratlong, M., Severac, D., Tejedor, G., Luz-Crawford, P., Nguyen-Chi, M., Mathieu, M., Jorgensen, C., Djouad, F. (2021) NRG1/ErbB signalling controls the dialogue between macrophages and neural crest-derived cells during zebrafish fin regeneration. Nature communications. 12:6336.
Abstract
Fish species, such as zebrafish (Danio rerio), can regenerate their appendages after amputation through the formation of a heterogeneous cellular structure named blastema. Here, by combining live imaging of triple transgenic zebrafish embryos and single-cell RNA sequencing we established a detailed cell atlas of the regenerating caudal fin in zebrafish larvae. We confirmed the presence of macrophage subsets that govern zebrafish fin regeneration, and identified a foxd3-positive cell population within the regenerating fin. Genetic depletion of these foxd3-positive neural crest-derived cells (NCdC) showed that they are involved in blastema formation and caudal fin regeneration. Finally, chemical inhibition and transcriptomic analysis demonstrated that these foxd3-positive cells regulate macrophage recruitment and polarization through the NRG1/ErbB pathway. Here, we show the diversity of the cells required for blastema formation, identify a discrete foxd3-positive NCdC population, and reveal the critical function of the NRG1/ErbB pathway in controlling the dialogue between macrophages and NCdC.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping