FIGURE

Figure 6

ID

ZDB-FIG-210930-13

Publication

Qi et al., 2021 - Reduced Acrolein Detoxification in akr1a1a Zebrafish Mutants Causes Impaired Insulin Receptor Signaling and Microvascular Alterations

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Figure 6

ACR induced alterations on retinal hyaloid vasculature and downregulation of insra/insrb mRNA expression at 5dpf. A) Representative confocal images of hyaloid vasculature. White scale bar: 20 µm. B,C) Quantification of hyaloid vasculature showed significant increasing numbers of branches in akr1a1a1^+/+ larvae incubated with 10 µm ACR. n = 13–16. White scale bar = 20 µm. D) Insa mRNA expression was unaltered in akr1a1a1^+/+ larvae upon ACR treatment. n = 7 clutches with 30 larvae. E) Whole‐body glucose measurement showed higher glucose level in akr1a1a1^+/+ larvae upon 10 µm ACR treatment at 5dpf. n = 4 clutches with 20 larvae. F,G) Both insra and insrb showed declined mRNA expression level in akr1a1a1^+/+ larvae upon 10 µm ACR treatment. n = 7 clutches with 30 larvae. For statistical analysis Student's t‐test was applied. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. NS, not significant.

Expression Data

Genes:	ins insra insrb
Fish:	y1Tg akr1a1a^{zf3750/zf3750}; y1Tg
Condition:	chemical treatment by environment: acrolein
Anatomical Term:	whole organism
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	y1Tg akr1a1a^{zf3750/zf3750}; y1Tg
Condition:	chemical treatment by environment: acrolein
Observed In:	blood glucose hyaloid vessel hyaloid vessel sprouting angiogenesis whole organism
Stage:	Day 5

Phenotype Detail

Acknowledgments

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