FIGURE

FIGURE 3

ID

ZDB-FIG-210817-7

Publication

Lin et al., 2021 - Base editing-mediated perturbation of endogenous PKM1/2 splicing facilitates isoform-specific functional analysis in vitro and in vivo

Other Figures

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FIGURE 3

PKM/E9‐GT mutation promotes cell growth and activates cell cycle–related gene expression. A, E9‐GT mutation promotes cell growth. CCK‐8 kit was used to analyse the cell growth of single‐clone–derived E9‐GT‐mutant cells and wild‐type HCT116 cells were used as a control. Absorbance was measured at OD450 at 0, 48 and 96 h. B, Wild‐type and E9‐GT‐mutant (PKM1 loss) cells were subjected to RNA‐seq analysis, and differentially expressed genes (upregulated and downregulated) were presented as a heatmap. Representative differentially expressed genes were also presented. C, The relative expression of differentially expressed genes was analysed from FPKM values in RNA‐seq data. The mean values from 3 single‐clone–derived E9‐GT‐mutant cell lines were shown. D, GO analysis of the differentially expressed genes upon E9‐GT mutation. E, KEGG pathway analysis of the differentially expressed genes upon E9‐GT mutation

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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