FIGURE

FIGURE 1

ID

ZDB-FIG-210817-23

Publication

Lin et al., 2021 - Base editing-mediated perturbation of endogenous PKM1/2 splicing facilitates isoform-specific functional analysis in vitro and in vivo

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FIGURE 1

Base editing‐mediated efficient mutation of splicing junction sites of PKM gene. A, Schematic of ABEmax‐NG induced T‐to‐C in PKM gene. PKM1 contains exon 9 in yellow square, and PKM2 contains exon 10 in red square. The gRNA sequence is underlined in black and the PAM sequence is underlined in red. The targeted splicing sites are highlighted in red. B‐C. The mutation efficiencies of ABEmax‐NG‐induced T‐to‐C in E9‐GT B, or E10‐GT C, mutant cells were analysed from Sanger sequencing data using EditR

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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