ZFIN Image: Lin et al., 2021, FIGURE 3

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Figure Caption

FIGURE 3

PKM/E9‐GT mutation promotes cell growth and activates cell cycle–related gene expression. A, E9‐GT mutation promotes cell growth. CCK‐8 kit was used to analyse the cell growth of single‐clone–derived E9‐GT‐mutant cells and wild‐type HCT116 cells were used as a control. Absorbance was measured at OD450 at 0, 48 and 96 h. B, Wild‐type and E9‐GT‐mutant (PKM1 loss) cells were subjected to RNA‐seq analysis, and differentially expressed genes (upregulated and downregulated) were presented as a heatmap. Representative differentially expressed genes were also presented. C, The relative expression of differentially expressed genes was analysed from FPKM values in RNA‐seq data. The mean values from 3 single‐clone–derived E9‐GT‐mutant cell lines were shown. D, GO analysis of the differentially expressed genes upon E9‐GT mutation. E, KEGG pathway analysis of the differentially expressed genes upon E9‐GT mutation

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