FIGURE

Fig 8

ID

ZDB-FIG-210813-20

Publication

Gonçalves-Carneiro et al., 2021 - Origin and evolution of the zinc finger antiviral protein

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Fig 8

Differences in the RNA binding profiles of human and chicken ZAP.

(A) HEK293T ZAP^-/- and TRIM25^-/- cells were transfected with an HIV-1_CG proviral plasmid and a plasmid expressing human ZAP or chicken ZAP chimera. Cells were treated with 4-thiouiridine prior to UV-crosslinking and CLIP analysis. Reads associated with human (top) or chicken (bottom) ZAP were mapped to the HIV-1_CG genome and the read density plotted against position in the HIV-1_CG genome. CpG-enriched region the HIV-1_CG genome is highlighted in red and dashed lines. (B) Contribution (% observed/expected) for each dinucleotide to reads derived from the HIV-1_CG genome to was calculated. Shift in the ratio for each dinucleotide contribution comparing human and chicken ZAP reads was also determined (blue line).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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