FIGURE

Fig 7

ID

ZDB-FIG-210813-19

Publication

Gonçalves-Carneiro et al., 2021 - Origin and evolution of the zinc finger antiviral protein

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Fig 7

Avian ZAP proteins are less selective for CpG enriched HIV-1.

(A-B) HEK293T ZAP^-/- and TRIM25^-/- cells were co-transfected with indicated proviral plasmids, increasing amounts (0ng, 75ng, 145ng, or 290ng) of a plasmid encoding an FLAG-tagged human ZAP or mouse, bat, chicken and alligator ZAP chimeras and a fixed amount of a plasmid encoding a cognate HA-TRIM25 protein. After 48h, infectious virus yield was determined using MT4-R5-GFP target cells (A). Whole cell lysates were analysed by western blotting probing with antibodies against HIV-1 proteins ZAP-FLAG and TRIM25-HA (B). (C) HEK293T ZAP^-/- and TRIM25^-/- cells were transfected with HIV-1 proviral plasmids, along with increasing amounts (0ng, 75ng, 145ng, or 290ng) of a plasmids expressing human ZAP or ZAP chimeras from various avian species. For cells transfected with human ZAP, a fixed amount of a human TRIM25 expression plasmid was co-transfected, while for the avian ZAP chimeras, chicken TRIM25 was used. After 48h, infectious virus yield was determined using MT4-R5-GFP target cells.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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