FIGURE

Fig 4

ID

ZDB-FIG-210813-15

Publication

Gonçalves-Carneiro et al., 2021 - Origin and evolution of the zinc finger antiviral protein

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Fig 4

ZAP protein antiviral activity in human cells.

(A) Schematic representation of ZAP chimeras in which the RNA binding domain of mouse, bat, chicken and alligator ZAP was fused to the WWE and PARP-like domain of human ZAP. (B-C) HEK293T ZAP^-/- cells were transfected with HIV-1_WT (W) or HIV-1_CG (C) proviral plasmids and increasing amounts (0ng, 75ng, 145ng, or 290ng) of a plasmid encoding each ZAP chimera. Supernatant was harvested after 48h and the infectious virus yield was determined using MT4-R5-GFP target cells (B). Whole cell lysates corresponding to the highest amount of ZAP plasmids transfected were analysed by western blotting probing with antibodies against HIV-1 proteins and ZAP (C). -, Indicates co-transfection with an empty vector in place of a ZAP expression plasmid.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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