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C-terminal <italic>asxl1</italic> mutation leads to MDS like with neutrophilic dysplasia in adult zebrafish.A Proportion of hematopoietic cells in whole kidney marrow of 6-month-old asxl1+/+, asxl1−/− (n = 9 and n = 10, respectively; two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001; error bars, mean ± SD). Mye P myeloid progenitors, Mye mature myeloid cells, Ery erythrocytes, Lym lymphocytes, Neu neutrophils, Mac macrophages, Eos eosinophils. May–Grünwald–Giemsa staining of whole kidney marrow from 6-month-old asxl1+/+ and asxl1−/− (scale bar, 20 μm; black boxes show enlarged details of neutrophils; red arrows, neutrophils). B May–Grünwald–Giemsa staining of neutrophils from kidney marrow. Neutrophils were quantified by morphology. Neutrophils were collected from ten adult fish of each genotype asxl1+/+ or asxl1−/−. After staining, 500 cells were randomly chosen for further calculation. This process was repeated three times for statistical tests (scale bar, 20 μm; black boxes show enlarged details of neutrophils; red arrows, neutrophils; two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001, error bars, mean ± SD). C qRT-PCR comparing expression of myeloid markers in sorted neutrophils from asxl1+/+ and asxl1−/−. Neutrophils were collected from ten adult fish of each genotype asxl1+/+ or asxl1−/−. qPCR was performed with three technical replicates (two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001, error bars, mean ± SD) (color figure online).
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