asxl1 mutated cells intrinsically induce leukemogenesis and are transplantable.A Schematic of the experimental procedure. Wild-type fish were irradiated at 24 Gy 2 days before transplantation. Whole kidney marrow cells were prepared from 1-year stage asxl1+/+ and asxl1−/− donors and were dilute to 105/μL. Each recipient was injected with 3 μL donor kidney marrow cells. Transplanted fish were raised in sterile water. After 18 days, surviving fish were collected for analysis (dpt, days posttransplantation). B May–Grünwald–Giemsa staining of whole kidney marrow of successful transplanted fish (asxl1+/+, n = 3; asxl1−/−, n = 4; scale bar, 20 μm; black boxes show enlarged details of neutrophils and macrophages/monocytes; green arrows, neutrophils; red arrow, macrophages). C Proportion of hematopoietic cells in whole kidney marrow of transplanted fish (asxl1+/+, n = 3; asxl1−/−, n = 4; two-tailed Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001, error bars, mean ± SD). Mye P myeloid progenitors, Mye mature myeloid cells, Ery erythrocytes, Lym lymphocytes, Neu neutrophils, Mac macrophages, Eos eosinophils (color figure online).
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