FIGURE

Fig 5

ID

ZDB-FIG-210802-17

Publication

Wen et al., 2021 - The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics

Other Figures

All Figure Page

Back to All Figure Page

Fig 5

Knockdown of <italic toggle='yes'>klf6a</italic> impairs junction remodeling and rearrangement of actin cytoskeleton in CV pruning.

(A) and (B) Time-lapse live imaging of junction remodeling and actin cytoskeleton dynamics in CV pruning in Tg(Fliep:Lifeact-EGFP);KI(cdh5-mRFP) embryos. (A) Control embryo shows a retraction of junction (white arrowhead) and a shrinkage of its junctional ring (white and blue arrowheads) as multicellular tube became stenotic. F-actin forms at cdh5-positive junction (white, blue and yellow arrowheads), and undergoes a similar rearrangement as cdh5-positve junctions. 7 vascular loops are taken time-lapse live imaging. Scale bar: 25 μm. (B) Although klf6a morphant show F-actin co-localization with junction, however, neither junction nor F-actin does not go through remodeling (arrowhead). 5 vascular loops are taken time-lapse live imaging. Scale bar: 25 μm.

Expression Data

Genes:	EGFP mRFP
Fish:	cdh5^cqu2Tg; cqu6Tg cdh5^cqu2Tg; cqu6Tg + MO1-klf6a
Knockdown Reagent:	MO1-klf6a
Anatomical Terms:	caudal vein plexus cell-cell junction caudal vein plexus filamentous actin
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cdh5^cqu2Tg; cqu6Tg + MO1-klf6a
Knockdown Reagent:	MO1-klf6a
Observed In:	caudal vein plexus actin cytoskeleton organization caudal vein plexus blood vessel remodeling caudal vein plexus cell-cell junction maintenance
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.