FIGURE

Fig 6

ID

ZDB-FIG-210802-18

Publication

Wen et al., 2021 - The blood flow-klf6a-tagln2 axis drives vessel pruning in zebrafish by regulating endothelial cell rearrangement and actin cytoskeleton dynamics

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Fig 6

<italic toggle='yes'>Tagln2</italic> is a direct downstream target of <italic toggle='yes'>klf6a</italic>.

(A) and (B) SiKLF6 in HUVECs efficiently decrease TAGLN2 expression at the mRNA (KLF6: P = 0.0009, TAGLN2: P = 0.0352) and protein (KLF6: P < 0.0001, TAGLN2: P < 0.0001) levels. Student’s unpaired two-tailed t test. *P < 0.05, ***P < 0.001. (C) WISH of the tagln2 gene of sibling and klf6a^-/- zebrafish at 36 hpf. Arrowheads indicate the CVP region expressing tagln2. (D) Structure of the tagln2 promoter. The primer tagln2-F1+R1 contains the conserved Klf6a binding side-CACCC, whereas tagln2-F2+R2 is a negative control located in the tagln2 exon 1. (E) ChIP-PCR of the tagln2 promoter shows that Klf6a can bind directly to the tagln2 promoter.

Expression Data

Gene:	tagln2
Fish:	WT klf6a^cqu3/cqu3
Anatomical Term:	caudal vein plexus
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	klf6a^cqu3/cqu3
Observed In:	caudal vein plexus
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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